Accelerating the drug development process with Bruker high-throughput mass spec solutions powered by timsTOF technology and Bruker SPR Pro: Multiple ways of kinetic profiling with High Throughput Surface Plasmon Resonance
Senior Applications Scientist Bruker Daltonics GmbH & Co. KG, Germany
This tutorial will highlight the unique capabilities of Bruker's high-throughput pharma and biopharma mass spec solutions based on innovative timsTOF technology. The presentation will address various critically important drug discovery/development workflows and illustrate how Bruker's customized timsTOF solutions stand out to accelerate and enhance these, e.g. - Label-free (ultra)high-throughput screening and high-throughput experimentation monitoring in early drug discovery enabled by timsTOF MALDI PharmaPulse® - Molecular imaging in tissues with MALDI guided SpatialOMx® - High-throughput protein degradation studies, HCP analysis and immunopeptidomics facilitated by 4D-Proteomics™ Ecosystem - Complete characterization of DNA, RNA and beyond provided by OligoQuest™ Surface Plasmon Resonance is an established and widely used biophysical technology in screening and lead development campaigns for novel drug candidates. The real-time, label-free analysis of interactions offers additional insights into kinetics as well as binding profiles. Besides the usage of a common multi injection kinetic, the titration cycle kinetic offers a quick insight into the kinetics behavior of a full screening campaign. We herewith present an automated and high-throughput optimized approach for kinetic profile determination using SPR. Especially the high comparability between the shown approaches will increase the flexibility for the user to determine the typical parameters from a SPR experiment.