Well-validated chemical modulators of protein function, so-called chemical probes, are essential tools for our understanding of the biological and therapeutic relevance of a protein. The SGC, a public-private partnership has been developing these tools for many years and makes them available to the community. After an initial focus on Epigenetic targets, we have expanded the effort to generate specific tools for a variety of proteins, including kinases and E3 ligases. More recently, we have complemented our chemical probe effort by programs assembling chemogenomic libraries, consisting of subsets of well-annotated compounds with narrow specificity and complementary selectivity to provide well-characterized tools for a significant part of the human proteome. In addition to extensive in vitro profiling, thorough cellular characterization is necessary. Available libraries, their characterizations, and examples of use in primary human organoids will be presented.
