(1094-A) Single-cell printing, and screening of cell lines for monoclonality verification using an impedance-based single-cell dispenser and high-throughput fluorescence-based imager.

Abstract: Single-cell isolation is critical in cell line development. It leads to stable, high-producing clonal cell lines, improving efficiency, safety and quality of applications like CRISPR, drug discovery, personalized medicine and antibody production. There is a need for a fast and efficient single-cell isolation solution that preserves cell integrity and clonality. High-throughput screening and automation can speed up the process. Our workflow includes a single-cell dispenser (DISPENCELL®2) and a cell imager (Clone Select® Imager FL1). Our method demonstrates improved throughput and automation compared to limiting dilution method, with a single-cell printing efficiency of 60-90%. This impedance-based method isolates intact single cells and has potential for gene editing, generating engineered cell lines and facilitating bioprocess development or drug screening.